Expression profiling of purified cells after (mature) paneth cell depletion.

We used transgenic mice expressing diphtheria toxin in a paneth cell specific manner to eablate paneth cells, but found that we could still detect and also purify (paneth) cells in the transgenic mice. We purified this population and a control WT population of paneth cells for expression profiling, with the goal to check if the diphtheria toxin expression system had low penetrance or if our PC population in TG mice was immature , and thus did not yet express the active diphtheria toxin. Overall design: 1 group of wild type mice and one group of transgenic mice withe paneth cells expressing diphtheria toxin, with 4 replictes per group