Mean difference values and precision of gene expression in samples from patients with different BMI and HOMA-IR index.

The mean difference (MD) for each sample was calculated as the difference of Ct from RPL13A, EEF1A1, CYC, GAPDH and 18S to the average of the other four genes and tissues. Then, the difference was averaged separately for each group (Lean L-IR, Lean H-IR, MO L-IR, MO H-IR). The right column represents the mean values for the four groups. The precision was calculated as 2-fold standard deviation (±2SD). No statistical differences for MD were found among groups (one-way ANOVA). Expression levels for VAT were 18S>EEF1A1>CYC>RPL13A>GAPDH, and those of SAT were 18S>EEF1A1>RPL13A>CYC>GAPDH. Precision ranged from 1.39 for RPL13A to 2.86 for 18S in VAT and from 1.67 for EEF1A1 to 2.85 for GAPDH in SAT. VAT: visceral adipose tissue; SAT: subcutaneous adipose tissue. Lean L-IR: lean subjects with low-degree insulin resistance; Lean H-IR: lean subjects with high-degree insulin resistance; MO L-IR: morbid obese subjects with low-degree insulin resistance; MO H-IR: morbid obese with high-degree insulin resistance.