The intestinal microbiota of channel catfish (Ictalurus punctatus) fed different combinations of iron and copper in bulk or nanoparticle form.

Iron and copper were supplemented in the diet of catfish fingerlings in different combinations: inorganic iron + inorganic copper, only copper nanoparticles, only iron nanoparticles, and iron nanoparticles + copper nanoparticles. Fingerlings were fed for 60 days. The digesta from the whole intestine was sampled and subjected to DNA extraction, followed by sequencing the V3-V4 region of the 16S rRNA.