Cucumis melo chrarentais mono sequencing

Our work is a summary of an investigation that lasted five years. We report a full story that started with chromosome scale genome assembly of Cantaloupe melon and finished with the reconstruction of the ancestral Cucurbitaceae karyotype and compared it to sequenced cucurbit genomes. To sequence cantaloupe melon genome we used a combination of SMRT sequencing technology, Bionano optical mapping, Hi-C and 10x Genomics. Chromosome scale genome was assembled and genes and transposable elements annotated. As 3D chromatin features are central in the regulation of gene expression, we analyzed the chromatin architecture by integrating RNA-seq, Hi-C, and histone and DNA methylation marks. To bring new insight in melon genome evolution, we also investigated sequence divergence of sequenced melon botanical groups and the paleohistory of melon genome relative to constructed ancestral Cucurbitaceae karyotype. Our investigations, in addition to the sequence of the genomes of other melon botanical groups are expected to further help understanding the phenotypic diversity and cultivation of melon botanical groups and evolutionary history of cucurbits.