Recruitment of IRF3 to activated ZBP1:TBK1

ZBP1 (DAI) dimer formation enables recruitment of TBK1 and IRF3 to the C-terminal region of DAI in response to cytosolic DNA in murine L929 cells. This interaction is DNA-dependent as ZBP1(DAI) mutants that lack DNA binding domains neither recruited TBK1 nor activated IRF3 (Takaoka A et al 2007). Activation of IRF-3 and possibly IRF-7 promotes IFN gene expression.

ZBP1（DAI）二聚体形成使TBK1和IRF3能够募集至小鼠L929细胞中DAI的C端区域，以响应细胞质DNA。此相互作用依赖于DNA，因为缺乏DNA结合域的ZBP1（DAI）突变体既未能募集TBK1，也未激活IRF3（Takaoka A等，2007年）。IRF-3的激活以及可能的IRF-7激活，促进IFN基因的表达。