MOESM1 of Design and evaluation of nano-hydroxyapatite/poly(vinyl alcohol) hydrogels coated with poly(lactic-co-glycolic acid)/nano-hydroxyapatite/poly(vinyl alcohol) scaffolds for cartilage repair

Additional file 1: Figure S1. Toluidine blue staining(A) and immunohistochemical staining of type II collagen(B) of chondrocytes; The growth curve of rabbit chondrocytes(C). G represents the cell-generation of rabbit chondrocytes passage number. Figure S2. Western-Blot analysis of Col2 and GAG protein expression and actin expression after co-culturing rabbit chondrocytes with scaffold for 3d,7d,14d. PVA mass fraction of 5wt%(E1), 10wt%(E2), or 15wt%(E3); PLGA of 30wt%; and nano-HA of 5wt% was used for investigation. The control group contained HA: 5wt%, PVA: 15wt% (CG). Figure S3. Results of toluidine blue staining after co-culturing rabbit chondrocytes with scaffold for 3d,7d,14d; PVA mass fraction of 5wt%(E1), 10wt%(E2), or 15wt%(E3); PLGA of 30wt%; and nano-HA of 5wt% were used for investigation. The control group contained HA: 5wt%, PVA: 15wt% (CG). Figure S4. The bottom-emitting image of the double-layer HA/PVA hydrogel coated with PLGA/HA/PVA scaffold. The radius of mold 2 was 10 mm, and that of mold 1 was 7 mm in experimental group one (EG1), 8 mm in experimental group two (EG2), and 9 mm in experimental group three (EG3).