Iliff_et_al.

This data was collected for an undergraduate thesis project that is now being submitted for publication in Marine Pollution Bulletin.

Here we elucidated the potential chronic effects of the commonly utilized pesticide, carbaryl, on oyster reef communities in the Loxahatchee River Estuary in southeast Florida. Though carbaryl had a limited effect on total epifaunal community diversity, species richness and evenness, the results of this experiment indicate that carbaryl significantly shifted crustacean community composition, resulting in a substantial loss in total crustacean abundance. One crustacean in particular, Americorophium spp. (tube building amphipod), was significantly less abundant within the carbaryl treatment, driving the shift in crustacean community composition. Ultimately, our results signal that pesticide pollution in estuaries will negatively impact crustaceans.

Organisms were allowed to colonize a string of 5, 10 cm^2 travertine tiles for 28 days in the Loxahatchee River Estuary in Jupiter, Florida. For the data file "mobile fauna", all unattached organisms were rinsed off of the tiles, and rinsed in a 500 micron sieve and were stored in alcohol. The specimens were then sorted, identified to the lowest practical level and quantified. The most abundant species were small crustaceans including several species of amphipod. For density calculations, these organisms were collected from an area of 0.1 square meters.

For the file labeled "sessile_fauna", the organisms attached to the tiles were identified and quantified under a dissecting scope. Only the middle three tiles were analyzed this way from an area of 0.06 square meters.

For the data file labeled "dissolutionblockdata", we used plaster dissolution blocks to slowly release carbaryl insecticide adjacent to tiles. The initial and final weights of each block were recorded before deployment and after collection.

本数据集收集于一项本科毕业论文项目，该项目现正提交至《海洋污染通报》杂志发表。本研究阐释了广泛使用的农药卡巴肼对佛罗里达东南部洛哈奇河三角洲中牡蛎礁群落的潜在慢性影响。尽管卡巴肼对总附着生物群落多样性、物种丰富度和均匀度的影响有限，但实验结果指出，卡巴肼显著改变了甲壳类生物群落组成，导致甲壳类生物总数量显著减少。尤其是管状甲壳类生物Americorophium spp.（建造管状足目甲壳类），在卡巴肼处理组中的数量显著减少，从而推动了甲壳类生物群落组成的转变。最终，我们的研究结果警示，河口地区的农药污染将对甲壳类生物产生负面影响。

实验中，生物体被允许在佛罗里达州朱庇特洛哈奇河三角洲的5个、10平方厘米的石灰华瓷砖上定居28天。对于“移动生物”数据文件，所有未附着在瓷砖上的生物体均被冲洗干净，并通过500微米筛网冲洗，并储存于酒精中。随后，标本被分类、鉴定至最低实用级别并进行量化。最丰富的物种为小型甲壳类生物，包括多种足目甲壳类。为计算密度，这些生物体从0.1平方米的区域收集。

对于标注为“附着生物”的文件，附着在瓷砖上的生物体在解剖显微镜下进行鉴定和量化。仅分析中间三个瓷砖，从0.06平方米的区域收集。

对于标注为“溶解块数据”的文件，我们使用石膏溶解块在瓷砖附近缓慢释放卡巴肼杀虫剂。每个块在部署前和收集后的初始和最终重量均被记录。