Relative binding of IGF-II and the IGF-II analogues for the IGF-1R and IR-A.

aBinding affinities of the analogues relative to IGF-II were derived from the IC50 values. Relative binding is expressed as a percent of IGF-II ± S.E. The IC50 of IGF-II for each assay is shown in parentheses.
bBinding to R−IR-A = binding to R−IR-A cells, IR-A = to immunocaptured IR-A, P6 IGF-1R = to BALB/c3T3 cells overexpressing the IGF-1R, IGF-1R = to immunocaptured IGF-1R.
cR−IR-A/P6 IGF-1R is the ratio of IC50 values for binding to the R−IR-A cells versus P6 IGF-1R. Data is derived from at least 3 separate experiments performed in triplicate.
*** = p<0.001,
** = p value 0.001 to 0.01,
* = p value 0.01 to 0.05, ns = not significant when compared to IGF-II.