Activated NK cells with a predominance of inhibitory receptors and a decidual-like phenotype expand after autologous HSCT in children with tumors

In this study, we wanted to study the changes in the NK cell transcriptome after culturing in the presence of IL-15 and TGF-beta by using single cell RNA sequencing (sc-RNAseq) Overall design: Peripheral blood mononuclear cells were collected from a healthy donor and cultured in the presence of IL-15+TGF-beta or non-stimulus. Cells were cultured for 1 and 4 days. Next, sc-RNAseq was performed using Illumina Novaseq 6000.