Multi-omics approaches for revealing the epigenetic regulation of histone H3.1 during spermatogonial stem cell differentiation in vitro

Epigenetic regulation, particularly histone post translational modification (PTM), participates in spermatogonial stem cells (SSCs) differentiation. However, prior research is still limited to a single PTM. Herein, we constructed over 100 histone H3.1 targeted modification peptides, in combination with histone H3.1 modification binding protein identification, and RNA-Seq. We identified 7 differentially expressed histone H3.1 modifications. In addition, we selected H3K9me2 and H3S10ph for subsequent biotinylating peptide pull-down experiments, and identified a series of transcription factors, such as, GTF2E2 and SUPT5H, which appear to be crucial for the early SSC differentiation process. Comparative gene expression profiling analysis of RNA-seq data for three stages of SSCs cells differentiated in vitro.