Juvenile and adult sea Lamprey behaviour (twitch and movement)

Sea lamprey are invasive fish species in the Laurentian Great Lakes and parasitically feed on many commercially important fishes. Sound has been used as a deterrent for invasive species, but its potential for manipulating sea lamprey behaviour in natural stream conditions is under-tested. Both the behavioural acoustic threshold and differential responses at different life stages of sea lamprey have also yet to be established. To fill in some of these literature gaps, low-frequency tones of 70 or 90 Hz were used in a laboratory setting to determine the behavioural responses of adult and juvenile sea lamprey. Both stages of sea lamprey exhibited a change in swimming behaviour and a twitch (startle) in response to both frequencies. Adults had a lower threshold to 70 Hz than 90 Hz tone bursts with juveniles showing no difference between frequencies. These thresholds and behaviours can be used in natural settings to control or impact the behaviour of sea lamprey to aid in directing lamprey m..., Data was collected using experimental tones, of 70 or 90 Hz, played at varying amplitude levels (level 0-15 on the MP3 player, 129 – 169 dB re 1 µPa, -29.95 to -34.75 dB re 1 m/s2). Sea lamprey would need to be consistently still for 1 minute; the sound would then be played for 1 minute, and any behavioural reactions were recorded during the sound treatment. The behavioural reaction of the sea lamprey was also recorded directly after the sound was played. A stepwise method was used for decibel changes, with sea lamprey responses leading to a higher decibel level for no responses and lower decibel levels or a repeated decibel level for behavioural responses (movement). Analysis of all trials examined the proportion of time spent swimming, resting or attached to the tank with and without the presence of sound. 
Adult and juvenile sea lamprey behaviour was also compared by the immediate response to sound application. This “twitch” behaviour was quantified by tail or body movement that..., , # Juvenile and adult sea Lamprey behaviour (twitch and movement)

[https://doi.org/10.5061/dryad.5hqbzkhgt](https://doi.org/10.5061/dryad.5hqbzkhgt)

## Description of the data and file structure

### Files and variables

#### File: Juvenile\_Twitch\_Proportion.xlsx

**Description:** 22 trials were performed on juvenile sea lamprey, with 10 trials with 70 and 11 trials with 90 Hz tones and one trial including both frequencies. Juvenile sea lamprey behaviour of immediate response to sound application. This “twitch” behaviour was quantified by tail or body movement that occurred in the first second of sound presented. The behaviour of the sea lamprey was comparing the behaviour of the sea lamprey the minute directly before sound was played, as a baseline, and the response during the first second of sound which could be described as a “twitch.” Each trial, for each lamprey, has a row for each Level/Sound Pressure Level/Particle Acceleration even if that level was not tested in that trial, ...,