RNAseq profiling of dog primary mammary triple-negative breast cancer cells infected or not with a vaccinia virus deleted for the thymidine kinase gene

Dog breast cancer cells were infected at a multiplicity of infection of 5 for 6 hours or mock-infected (Control) for 6 hours prior to analyses by single-cell RNA sequencing. The objective was to study the distribution of host cell and viral gene expression.on the cancer cell population and to compare the effect of viral infection on these cells. RNA-seq profiling of uninfected and infected cells at different time points in 2 replicates