The effect of STING knockout on gene expression in cholestatic mice

STING has been identified to play a role in the onset and progression of various liver diseases.We aim to investigate whether STING mediates cholestatic liver injury. We here report that STING knockout significantly reversed cholestatic liver injury induced by bile duct ligation (BDL) and Mdr2 deficiency. Mechanistically, BDL induced the enrichment of several signaling pathways, including those associated with programmed cell death in the liver, type I interferon, innate immune response, chemotaxis, inflammation, senescence, and extracellular matrix assembly, which were significantly reversed by STING knockdown. Overall design: Total liver RNA is extracted from mice, which are subjected to bile duct ligation (BDL) for seven days or 60-day-old Abcb4 deficiency. Then, RNA sequencing analysis is performed.