Table_6.xlsx

Localized cutaneous leishmaniasis (LCL) is a chronic disease characterized by ulcerated skin lesion(s) and uncontrolled inflammation. The mechanisms underlying the pathogenesis of LCL are not completely understood, and little is known about posttranscriptional regulation during LCL. MicroRNAs (miRNAs) are non-coding small RNAs that regulate gene expression and can be implicated in the pathogenesis of LCL. We investigated the involvement of miRNAs and their targets genes in human LCL using publicly available transcriptome data sets followed by ex vivo validation. Initial analysis highlighted that miRNA expression is altered during LCL, as patients clustered separately from controls. Joint analysis identified eight high confidence miRNAs that had altered expression (−1.5 ≤ fold change ≥ 1.5; p

地方性皮肤利什曼病（LCL）是一种以溃疡性皮肤损害及炎症失控为特征的慢性疾病。LCL的发病机制尚不完全明了，关于LCL转录后调控的了解亦甚微。miRNA（微小RNA）是一种非编码的小型RNA，可调控基因表达，且在LCL的发病机制中可能起到重要作用。本研究采用公开可用的转录组数据集，通过体外验证，探讨了miRNA及其靶基因在人类LCL中的参与情况。初步分析表明，miRNA的表达在LCL过程中发生改变，患者组与对照组成员呈现明显分离。联合分析鉴定出八个高置信度的miRNA，其表达发生显著变化（变化倍数范围：-1.5≤fold change≥1.5；p值范围：未提供）。