Transcriptome response study of arabidopsis wild type (Col-0), ERF95 and ERF97 overexpression lines to basal heat stress

Analysis of transcriptome response of heat treated or untreated 7-day old whole seedlings with genotypes: Col-0 (wild type), p35S:ERF95 overexpression line and p35S:ERF97 overexpression line. ERF95 and ERF97 are involved in heat stress response. Results provide insight into the nuclear genes expression profile under heat treatment in Col-0, the nuclear genes expression profile regulated by either ERF95 and ERF97 overexpression before and after heat treatment, and the regulation similarity between ERF95 and ERF97 overexpression lines. Overall design: Total RNA samples were isolated from 7-day old whole seedlings with genotypes Col-0 (wild type), ERF95 and ERF97 overexpression lines grew under 16 h/8 h light/dark cycle condition with 100 µmol.m-2.s-1 light intensity at 22 °C following with or without 43 °C 22 min heat treatment. mRNA expression profiles were generated by deep sequencing, in duplicate, using Illumina HiSeq 2500.