Highthroughput epigenetic sequencing of ATAD2 for for cancer stem cells (CSCs) in clear cell renal cell carcinoma (ccRCC).

Here we identify a subset of vascular pericytes, defined by expression of GPR91, that promote tumorigenesis and tyrosine kinase inhibitors resistance by functioning as the primary methionine source for cancer stem cells in clear cell renal cell carcinoma. Cancer stem cells use methionine to create stabilizing N6-methyladenosine in ATAD2 mRNA, and the resulting ATAD2 protein complexes with SOX9 to remodel chromatin. Thus, in this study, we conducted the ChIP-seq, ATAC-seq, RNA-seq to depict the epigenetic roles of ATAD2 in CSCs.