2D culture characterisation of potential markers in human HER2-positive breast cancer cell lines

This dataset characterises the changes in 2D breast cancer models of hallmark gene expression under basal growth conditions and cell proliferation in the added heparin cultures. To validate this purpose, two human breast cancer cell lines (SKBR3 and MDA-MB-453), represented HER2-positive breast cancer, were grown under the basal growth media to 80% confluence. For the hallmark gene expression profiles, cells were harvested the total RNA, and RNA was converted to cDNA for gene expression examined using Q-PCR. Gene expression panels include fibroblast growth factor, receptors, cytokeratins and WNT signalling pathway components. For the cell proliferation changes, the two breast cancer cells were grown in 24-well plates treated with varying concentrations of heparin; cells were then collected and counted from day 1 to day 7. The total live cell was examined by the cell hemacytometer. For this dataset, cells were grown in biological triplicate and the experiment was taken in technical quadruplicate. Researchers in cellular biology and oncology will benefit from these data.