Dietary interventions modulate global non-coding RNA expression: a role for microRNA-chromatin-mRNA crosstalk [Diet_mRNA]

Using high-throughput deep sequencing, both coding and long non-coding RNA expression was profiled across six dietary intervention groups, including low fat (LF) or high fat (HF) with or without calorie restriction (LF+CR, HF+CR) or exercise (LF+Ex, HF+Ex). Overall design: Mice were treated starting at 5 weeks of age with LF or HF diet with or without CR or Ex. Liver RNA was extracted and used for polyA+ RNA-sequencing at midlife (62 weeks of age).