Analysis of m6A methylome in ABRO1 Knockout heart

To find the m6A methylation targets of ABRO1, we performed m6A methylated RNA immunoprecipitation sequencing (MeRIP-seq) in ABRO1 Knockout or Wild type (WT) mice heart. The analysis of the distribution of m6A peak density in mRNA transcripts showed that m6A peaks are mainly found in coding sequences (CDS) and a considerable amount of m6A peaks enriched around start codon and stop codon regions in mRNA transcripts from ABRO1 KO and WT hearts. Among the total RNA transcripts with m6A sites, more than half (59.4%) of mRNA transcripts contain = 2 m6A peaks, 27.4% mRNA transcripts comprise 3 to 5 m6A peaks and more than 5 m6A peaks exist in 13.2% of mRNA transcripts. MeRIP-seq analysis results from ABRO1 deleted hearts showed that a total of 3444 m6A peaks were upregulated and 4631 m6A were downregulated compared to WT hearts. Overall design: Total RNAs were extracted from ABRO1 Knockout or Wild type (WT) mice heart. MeRIP was performed to enrich m6A methylated mRNAs using anti-N6-methyadenosine (m6A) antibody. We used commercial kit (KAPA Stranded mRNA-seq Kit) for RNA-seq library preparation of both m6A enriched RNAs and input mRNAs. Then, high throughput RNA sequencing was performed to identify differences in the gene expression profile. Please note that each processed data was generated from both input and IP samples, and is linked to the corresponding IP sample records.