Mean percentages of bacterial viability in PL-biofilm, as well as intra-mouthrinse and inter-mouthrinse comparisons.

Mean percentages of bacterial viability in PL-biofilm under basal conditions and in the samples collected at 30 seconds and 1, 3, 5, and 7 hours after a single mouthrinse of sterile water and 0.2% Chlorhexidine differentiating between the 3 biofilm layers, as well as intra-mouthrinse and inter-mouthrinse comparisons.
– Not a statistically significant difference; M-water  =  a single, 30-second mouthrinse with 10 mL of sterile water; M-0.2% CHX  =  A single, 30-second mouthrinse with 10 mL of 0.2% Chlorhexidine; BASAL = Biofilm sample collected under basal conditions; 30 SEC  =  Biofilm sample collected at 30 seconds after the application of the different mouthrinses; 1 H  =  Biofilm sample collected 1 hour after the application of the different mouthrinses; 3 H  =  Biofilm sample collected 3 hours after the application of the different mouthrinses; 5 H  =  Biofilm sample collected 5 hours after the application of the different mouthrinses; 7 H  =  Biofilm sample collected 7 hours after the application of the different mouthrinses; the biofilm maximum thickness of each field was divided into 3 zones or equivalent layers: outer layer (layer 1), middle layer (layer 2) and inner layer (layer 3).