Acute atopic dermatitis and urticaria imprints peripheral monocytes with proinflammatory-prone signature

Monocytes from patients with type 2 immune disorders exhibited an augmented inflammatory response upon LPS stimulation. RNA-seq analysis demonstrated significant upregulation of inflammatory cytokines, including IL-6, IL-12, and IL-1b. Moreover, there was a conspicuous enrichment of signaling pathways associated with inflammation. ATAC sequencing revealed heightened chromatin accessibility in patient monocytes, particularly in the promoter regions of inflammatory genes. Monocytes in peripheral blood mononuclear cells (PBMCs) from patients diagnosed with atopic dermatitis and urticaria were collected for ATAC-seq