Larval and fat-body lipidomics of Drosophila CerS Schlank variants under normal and high-sugar diets

This dataset contains shotgun lipidomics measurements from male third-instar Drosophila melanogaster larvae and isolated larval fat bodies under normal diet (ND; 0.15 mol/L sucrose) and high-sugar diet (HSD; 1.0 mol/L sucrose). The knock-in experiment compares KIWT, KIE118A and KINLS1. The fat-body-specific allele-switch experiment compares r4>Sw WT→WT, r4>Sw WT→H215D and r4>Sw WT→NLS2. Each genotype–diet combination contains four biological replicates per tissue, with one larva or one fat body per replicate. Four Excel workbooks provide 96 sample profiles in total. They contain exported lipid signals and annotations, lipid-species quantities in pmol per larva or fat body, lipid-class totals and dimensionless Z scores. Z scores were calculated as (x − mean) / standard_deviation. The README describes the experimental design, worksheets, units, data fields and analytical method. The sample metadata table assigns unique identifiers and maps each sample to its columns in the four worksheet types. Instrumental acquisition files and separate quantitative-matrix CSV exports are not included in this version.