Guizotia abyssinica cultivar:noug Genome sequencing and assembly

GBS clustering, alignment and SNP discovery: Clustering of combined reads with CD-HIT-EST v4.6., allowing up to 5% difference, filtering of the clusters: excluding singletons and clusters created from < 20 reads. Alignment of subsampled quality-trimmed reads against cluster reference using Bowtie2 v2.2.3. Variant discovery and genotyping of samples with Freebayes v1.0.2-16. Filtering of variants using a GBS-specific rule set:the read count for a locus must exceed 8 reads. Filtering of parental alleles with regard to progeny Genetic distance matrix generation (Kosman/Leonard distance).