Binary Ti vectors pSiM24 and pSiM24-GUS/GFP conferred a kanamycin-resistant fresh weight (FW) increase in tobacco leaf discs after transformation with A. tumefaciens.

After the co-cultivation with A. tumefaciens strain GV3850 at 25°C for 3 days, leaf discs were selected on shooting medium containing 250 mg/L of kanamycin and 500 mg/L of cefotaxime for four weeks. Each treatment involved 5 plates with 10 leaf discs per plate. The same experiment was repeated two more times. Statistical analysis of the data was performed adopting one way ANOVA analysis (using GraphPad Prism version 5.01) and presented as the means ± S.D. A P value of less than 0.05 was considered significant indicated by different superscript letters.