SiRNA, shRNA sequence
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下载链接：
https://figshare.com/articles/dataset/SiRNA_shRNA_sequence/6489464
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资源简介：
HNSCC
cell lines PCI-4A/4B and PCI-37A/37B were supplied by the University of
Pittsburgh Cancer Institute (USA). These cell lines were cultured at 37 °C in a
humidified atmosphere of 5% CO<sub>2</sub> in the medium of Dulbecco's modified
Eagle's medium (DMEM; Invitrogen, Carlsbad, CA, USA) supplemented with 10%
fetal bovine serum (FBS; Gibco, Carlsbad, CA, USA), 100U/ml penicillin G and
100U/ml streptomycin. The RNA oligonucleotide miR-92b mimics, inhibitor, SP1
siRNA, and negative control were synthesized by Genepharma (2603, Genepharma, Shanghai,
China). MiR-92b inhibitor and negative control plasmid were purchased from
GeneChem (PMDE249003218, GeneChem, Shanghai, China). Transfection agent
Lipofectamine 2000 (11668500, Invitrogen) was used according to the
manufacturer’s protocol. Cells were harvested 24 hours after transfection for
cell migration, invasion, proliferation and wound healing assay, 48 hours after
for RNA isolation and 72 hours after for western blot analysis.This is the
sequences of siRNA and shRNA used in this study.
提供机构：
figshare
创建时间：
2019-02-08
