Next Generation Sequencing Facilitates Quantitative Analysis of Transcriptomics in children with Cryptorchidism

Cryptorchidism is the most common congenital defect in children's genitourinary system. It is one of the risk factors for male infertility and testicular tumors. The occurrence of cryptorchidism is closely related to many factors, and the alteration of genes plays an important role in the occurrence of cryptorchidism. A large number of studies have confirmed that the formation of cryptorchidism is related to the differential expression of genes. Therefore, exploring new differential expression genes of cryptorchidism from the transcriptome level and studying the relationship between susceptibility genes and children with cryptorchidism can provide a new direction for studying the pathogenesis of cryptorchidism. In order to check the difference between the genome-wide transcriptional profile of children with cryptorchidism and in normal control testis, we separately examined testicular tissue including the samples of 6 cases of children with cryptorchidism and 2 cases of testicular trauma children and 1 case of incarcerated inguinal hernias. The transcriptome sequencing of the 9 testicular tissues was performed by RNA-seq technique.