Microbiome of different gut regions after nutritional programming

The aim of this study is to investigate the impact of NP using either plant (V) diet in comparison to marine (M) diet (as control) on the microbiota of three gut regions (pyloric caeca, middle and distal intestines) in Atlantic salmon. A 22-week feeding experiment was conducted in a recirculating aquaculture system with fish fed one of two diets (M or V) as a first feeding nutritional stimuli for two weeks before being switched exclusively to a M diet for 14 weeks and later challenged with V diet for six weeks. Amplicon (16S rRNA) sequencing was used to investigate the changes in the bacterial composition across various regions of the fish gut to identify any potential differences following nutritional programming.