Phospholipase immobilized in the hydrolysis of lipid fraction of the wastewater slaughterhouse swine: comparison with enzyme free

ABSTRACT This study intended to determine the best operating conditions for the hydrolysis of lipids present in wastewater of slaughterhouse swine comparing a free commercial phospholipase and an immobilized one, as well as the potential for reuse of the immobilized phospholipase in hydrolysis reactions and the maintenance of its lipolytic capacity in storage conditions. The influence of temperature, pH and concentration of phospholipase hydrolysis were analyzed, yielding optimal values such as 36ºC, 8.5 and 1.1% (w.v-1), respectively. The amounts of free fatty acids obtained for free and immobilized enzyme differed significantly (p