Optimizing standardized lab-grown skin substitutes reveals a proliferation-differentiation switch based in ascorbic acid

The project "From 2D to 3D: Optimization of lab-grown skin substitutes reveals a physiological switch based on ascorbic acid." aimed to provide culture conditioning and adjusting of keratinocytes intended to be used in differentiation processes. The keratinocytes were evaluated on their capacity to generate competent skin constructs as a model of cellular differentiation. The influence of ascorbic acid in 2D and 3D epidermal models was characterized, as it was found to act as a physiological switch.