Small RNAs from the moss Physcomitrella patens. Physcomitrium patens

High-throughput sequencing of endogenous small RNAs from the moss Physcomitrella patens. This dataset encompasses microRNAs and other small RNAs of ~20-24 nucleotides expressed in the moss P. patens. SAMPLES UPDATED JULY 9, 2007 TO INCLUDE DATA ON SEQUENCED SMALL RNAS THAT DO NOT MATCH THE P. PATENS GENOME Keywords: High throughput small RNA sequencing Overall design: Small RNA cDNA libraries derived from polyacrylamide gel electrophoresis (PAGE) fractionated total RNA, adapter ligation, reverse transcription, and pyrosequencing was used for microRNA and endogenous siRNA discovery in wild-type tissues of the moss Physcomitrella patens. See Axtell et al. (2006) A two-hit trigger for siRNA biogenesis in plants. Cell 127: 565-577. for details.