The MTCY428.08 Gene of Mycobacterium tuberculosis Codes for NAD(+) Synthetase
收藏PubMed Central2026-05-16 收录
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https://pmc.ncbi.nlm.nih.gov/articles/PMC107825/
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资源简介：
The product of the MTCY428.08 gene of Mycobacterium tuberculosis shows sequence homology with several NAD(+) synthetases. The MTCY428.08 gene was cloned into the expression vectors pGEX-4T-1 and pET-15b. Expression in Escherichia coli led to overproduction of glutathione S-transferase fused and His(6)-tagged gene products, which were enzymatically assayed for NAD synthetase activity. Our results demonstrate that the MTCY428.08 gene of M. tuberculosis is the structural gene for NAD(+) synthetase.
提供机构：
American Society for Microbiology (ASM)
