4.4. Stemness markers evaluated by flow citometry after dissociation of Hydrogel Microbeads Seeded with 1 Million/mL Human Adipose-Derived Mesenchymal Stem/Stromal Cells, produced with Bioinks 2 and 3 via Inkjet Bioprinting Using a 50 µm Nozzle and Cultured for 21 Days.

Stemness markers evaluated by flow citometry after dissociation of Hydrogel Microbeads Seeded with 1 Million/mL Human Adipose-Derived Mesenchymal Stem/Stromal Cells, produced with Bioinks 2 and 3 via Inkjet Bioprinting Using a 50 µm Nozzle and Cultured for 21 Days. Inkjet bioprinting parameters: Nozzle diameter: 50 µm. Aperture time: 10 ms. Jetting pressure: 4 bar. Inkjet device temperature: 37°C. Sample codes: FAG 242: bioink 2 FAG 251: bioink 3 NM: non-marquées (cells without labelling, used to calibrate the citometer). Mix: mix of positive markers. iso: isotype internal control to calibrate unespecific signals. viab: viability sample labelled with annexin (labels apoptotic cells) and propidium iodide (labels necrotic cells). Monomarquage: samples labelled with only one marker to compensate internal citometer signals.