To characterize the DMTF1 proximity proteome using BioID-mass spectrometry analysis

Neural stem cells (NSCs) contribute to brain development and homeostasis via their unique properties of self-renewal and differentiation. DMTF1 is a transcription factor that is proposed to be a tumor suppressor in cancer cells. However, the exact role of DMTF1 in NSC biology remains poorly understood. We found that DMTF1 depletion reduced the proliferation/self-renewal of mouse and human NSCs in vitro. To elucidate how DMTF1 may regulate gene transcription along with other proteins, we have employed BioID-mass spectrometry analysis of DMTF1 vs GFP proximity proteomes in 293T cells. The identified candidate DMTF1 protein interactors will subsequently be validated in mouse NSC. This study aims to investigate how DMTF1 regulates NSC proliferation.