Transmittance and bidirectional reflectance changes induced by blue light in Arabidopsis wild type and chloroplast movement mutants

Reflectance single polarizer setup: Time courses of changes in red-light bidirectional reflectance induced by 1 h irradiation with low (0.1 µmol·m-2·s-1) or high (100 µmol·m-2·s-1) blue light of the adaxial surface of rosette leaves of 5-week-old Arabidopsis plants. Measurements were recorded for wild-type (WT), glabra1, phot1, phot2, phot1phot2, and jac1 mutants. The angle of incidence of the measuring and actinic beams was 40° or 55°. The detector was positioned at the angle of 0° (the detector normal parallel to the leaf normal) or either -40° (when incidence at 40°) or -55° (when incidence at 55°). The transmission axis of the polarizer was either parallel (P) or perpendicular (S) to the plane of incidence.
Reflectance two polarizer setup: Time courses of the polarized red-light bidirectional reflectance induced by 1 h irradiation with high blue light (100 µmol·m-2·s-1) of the adaxial surface of rosette leaves of 5-week-old wild-type and phot1phot2 Arabidopsis plants. The blue actinic and red measuring beams impinged the adaxial leaf surface at the angle of 55°. For measurements of diffuse reflectance, the detector was positioned at 0° to the leaf’s normal. For measurements of combined specular and diffuse reflectance, the detector was positioned at -55°. Four possible combinations of the orientation (P/S) of the polarizers in front of the measuring light source and the detector were examined
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