Diversity of vaginal microbiome during recurrent vulvovaginal candidiasis

Diversity of vaginal microbiome during recurrent vulvovaginal candidiasis. [Method]The amplicon libraries were constructed using Polymerase Chain Reaction (PCR) primers 341F and806R, which target the V3-V4 regions of the 16S rRNA gene. Primers ITS1F and ITS2 were used to amplify theITS1 region of the fungus. All primers were barcode-indexed and compatible with Illumina sequencing. All PCR reactions were conducted using a high-fidelity Phanta DNA polymerase according to the manufacturer's protocol (VAZYME, China) with a 100 ng of bacterial DNA being used as templates per reaction. [Group] [Control: health]: CAY,CLJ,CSW,WYF,ZQQ,ZSY,ZYL,XXD,MRY,NJD,LLL,LMY,LQD,JXM,HZ,DZY,DWF,CYY2; [Disease: vulvovaginal candidiasis]: CAM,CQS,CSF,CW,SDF,SLT,ZJT,ZJY,YXH,YSJ,XWZ,WFY,WJM,LZX,HMM,HXS,CYY1,CYF; [vulvovaginal candidiasis after Clotrimazole treatment]: FJF,GS,HXJ,JFZ,JYY,LF,LJJ,LXL,SMQ,XHM,YLY1,YSL,YSR,ZCE,ZF,ZJD,ZLR,ZXL.