mRNA transcription of candidate genes for thermal adaptation in the crimson-spotted rainbowfish.

Total mRNA was extracted from laboratory bred and reared individuals (F1); parents were wild-caught and originated from the Brisbane River. At 6 months of age, individuals from each family group were subjected to one of two thermal treatments for a duration of 80 days. Rearing tank (Tank), sex (Sex), thermal treatment group (Treat) individual identifier (animal), parents (DAM & SIRE) and full-sib family group (Fam) are provided for each individual. Immediately following thermal treatment, fish were euthanized and total mRNA extracted from liver tissues.

Transcript abundance was estimated via quantitative real-time PCR. LinRegPCR was used to convert raw CT values into estimates of initial/starting mRNA concentrations (N0). Initial mRNA concentrations were standardized relative to the geometric mean of two reference genes (geomean.REFS). Standardized values of candidate genes occupy the final 12 columns; gene symbols (column headings) are the same as those used in the accompanying article (McCairns et al. 2016).