CReSCENT: CanceR Single Cell ExpressioN Toolkit (CReSCENT) v2.0 PBMC example infiles and commands
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CReSCENT: CanceR Single Cell ExpressioN Toolkit (CReSCENT) v2.0
Example PBMC infiles and parameters
(c) Pugh Lab. Under GNU General Public License v3.0
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## CONTENTS
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A) File crescent_v2.0_pbmc_example_infiles_and_commands.tar.bz2,
folder INPUT_MTX contains four scRNA-seq PBMC datasets obtained from 10X
https://www.10xgenomics.com/resources/datasets/
Two of them originally had ~8,000 and ~10,000 cells, and were downsampled to 1,000
B) File crescent_v2.0_pbmc_example_infiles_and_commands.tar.bz2,
folder LISTS_AND_COMMANDS contains the tables with input parameters for the integration of the four
datasets above, using the four CReSCENT scRNA-seq one-line-command R scripts:
1) Runs_Seurat_v3_MultiDatasets_QC_Normalization.R
2) Runs_Seurat_v3_MultiDatasets_Integration.R
3) Runs_Seurat_v3_MultiDatasets_PCA_Clustering_DimReduction.R
4) Runs_Seurat_v3_MultiDatasets_DGE.R
Which can be obtained from:
https://github.com/pughlab/crescent/tree/master/bin/in_use
For help on how to run these scripts, in a Console/Terminal type:
`Rscript Runs_Seurat_v3_MultiDatasets_QC_Normalization.R -h`
Each script code has a section 'Required libraries'
For Bug Reports and Feature Requests, please fill out a GitHub ticket:
https://github.com/pughlab/crescent/issues
C) File crescent_v2.0_pbmc_example_infiles_and_commands.tar.bz2,
folder METADATA contains two tables with cell-level metadata to colour UMAP/TSNE plots
and to compare class_1 vs. class_2 for Differential Gene Expression detection
D) File crescent_v2.0_pbmc_example_outfiles.tar.bz2,
folder SEURAT contains the results obtained by running the scripts using CReSCENT scripts
GitHub version 5a78fe9.
See sub-folder 'SEURAT/LOG_FILES' for R library versions and commands used for each script
E) File crescent_gsva_pbmcs_example_infiles_and_commands.tar.bz2,
folder INFILES contains example infiles to run Runs_GSVA.R
F) File crescent_gsva_pbmcs_example_outfiles_and_commands.tar.bz2,
folder GSVA contains the results obtained by running script Runs_GSVA.R
folder LOG_FILES contains log files, including commands used for the run
G) File crescent_infercnv_glio_example_infiles_and_commands.tar.bz2,
folder INPUTS contains example infiles to run Runs_InferCNV.R
H) File crescent_infercnv_glio_example_outfiles_and_commands.tar.bz2,
folder INFERCNV contains the results obtained by running script Runs_InferCNV.R
folder LOG_FILES contains log files, including commands used for the run
创建时间:
2021-05-17



