potato transcriptomic

Nitrogen is an important nutrient for plant growth, its excess application leads to ultimately limit plant productivity and environmental damage excess. Potato is the third largest grain crop in the world. In its production process, there are problems such as large nitrogen fertilizer input and low utilization efficiency. Hence, improving nitrogen use efficiency of plant is one of the plausible options to solve the problems. In this study, the physiological indicators, transcriptome, and metabolome of two potato cultivars with contrasting NUE. The results showed that the N efficient genotype had greater chlorophyll and N content, more tuber yield, larger N fertilizer use efficiency, and higher N assimilation capacity than the N-inefficient genotype; Transcriptome analysis revealed that there were more differentially expressed genes in XS6 than NS7 in the low N treatment. That a large genetic variation existed in differentially expressed genes related to carbon and nitrogen metabolism between XS6 and NS7. Metabolome analysis identified 1252 metabolites in leaves and roots, which were divided into 10 classes. The metabolites up regulated in leaves and roots are mainly lipids and others. A nitrite reductase gene 102581851were up regulated by low N in XS6 and NS7, but XS6 is higher than NS7. Candidate structural genes related to N assimilation in nitrogen metabolism pathways were discovered by weighted gene co-expression network analysis. Conjoint analysis of the transcriptome and metabolome emphasized the importance of car-bon and nitrogen metabolism in enhancing NUE. Additionally, tricarboxylic acid cycle reduced in both potato varieties but more reduced in NS7.