Additional file 1 of Tick microbial associations at the crossroad of horizontal and vertical transmission pathways

Additional file 1: Table S1. Details on studied forest sites. Table S2. The qPCR protocol for detection of tick symbionts and pathogens. Table S3. Bacterial loads of samples for the 16S rRNA sequencing; sample scheme. Table S4. Details on a positive control, primers, protocol, and in silico analysis used in the 16S rRNA quantification analyses. Table S5. OTU table. Table S6. Prevalence of veritcally and horizontally transmitted symbionts and pathogens per location. Table S7. Expected and observed co-infections in questing nymphs and results of the exact binomial test. Table S8. Tick microorganism species-to-species associations (co-infections) in ticks.