Transcriptome profiling (RNA-seq) analysis of NaIO3-induced RPE cells with or witout CaPB treatment. Transcriptome profiling (RNA-seq) analysis of NaIO3-induced RPE cells with or witout CaPB treatment

Purpose: The goal of this experiment is to investigate the role of CaPB in NaIO3-induced RPE cells Methods: RPE cell mRNA profiles were generated by deep sequencing, in triplicate, using Illumina HiSeq 4000. Results: According to the differential expressions of mRNA genes, among which 463 genes were upregulated and 397 genes were downregulated with significance in NaIO3-induced RPE cells after CaPB treatment . From the Gene Ontology (GO), and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis, it has been identified that ferroptosis ranked as the most downregulated pathway upon CaPB treatment. Conclusions: Our study represented that CaPB against NaIO3-treated RPE cell death via ferroptosis inhibition Overall design: NaIO3-treated RPE with or without CaPB treatment were collected by deep sequencing, in triplicate, using Illumina HiSeq 4000.