Ifi27l2a regulates the microglial inflammation in the context of aging and stroke

Microglia are key mediators of inflammatory responses within the brain, as they regulate pro-inflammatory responses while also limiting neuroinflammation via reparative phagocytosis. To facilitate identification of potential mediators of inflammation, we performed single-cell RNA sequencing of aged mouse brains following stroke and found that Ifi27l2a was significantly up-regulated, particularly in microglia. Overall design: Sham and PDMCAO surgeries were performed on young and aged mice. At 14 days after surgery, brains were isolated. Both sexes were used for scRNA-seq. Fourteen days post PDMCAO (or sham surgery), anesthetized mice were transcardially perfused with heparinized PBS (10 U/mL). Brains were removed from the skull and sliced coronally into 3 mm-thick blocks (from a region spanning +1 to -2 mm from bregma), covering the cortical infarction and secondary thalamic injury site. The brain slice was then minced with a razor blade and subjected to the brain tissue dissociation protocol.The 10X Genomics Chromium™, Single-Cell RNA-SeqSystem (10X Genomics, Pleasanton, CA) was used to prepare cells for scRNA-seq.