Exercise improves age-related osteoporosis and cognitive impairment by regulating osteoblast-to-osteocyte transition

To validate the protection effect of physical exercises on age-related cognitive impairment, aged male C57BL/6J mice (12 months old, 12M) were subjected to treadmill running or standard housing for 4 weeks, and comparing with young (2 months old, 2M) male mice. For bone tissues, after collagenase A digestion for discarding the periosteum, the long bones are sagittal split apart followed by removing the bone marrow by extremely washing. All the bone species are subjected to mRNA isolation and bulk RNA-seq analysis. Comparative gene expression profilling analysis of RNA-seq data between 2M mice and 12M mice, or housing 12M mice and exercised 12M mcie.