Validation of microarray- and ChIP-chip-derived results by real-time PCR.
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Total RNA used for microarray analyses was subjected to real-time PCR quantification. The standardised expression ratio (SER) indicates the expression normalised to 18S rRNA and standardised to control condition (scrambled siRNA or vehicle). A SER of 100% indicates no expression difference versus control. P-values were based on a two-tailed, unpaired t-test. SEM: standard error of the mean with respect to the intervention; n: number of single PCR measurements. Fold changes are given in percent relative to the control condition based on the microarray data. Validated Genes, i.e., those which showed significantly (p
创建时间:
2015-12-02



