Summary of imprinted candidates identified in rice embryo and endosperm by transcriptome analyses and those confirmed by PCR.

1: Three embryo genes that met significance and four additional genes with suggestive evidence were analysed. Only one gene proved to be imprinted and it arose from the four additional genes analysed (Figure 1E). This gene is also imprinted in the endosperm.
2: Genes with ≥90% bias were tested.
3: Including 1 transcript from an intergenic region and five transcripts from intronic regions.
4: Maternal and paternal biased transcripts of different structure were predicted to originate from these genes as a result of novel alternative splicing. One locus was confirmed to show parent of origin-biased splicing (Figure 2 and Figure S5).