EPO/EPOR-modulated signal transduction factors.

Twelve additional signal transduction factors (STFs) were defined as significantly EPO/EPOR- modulated targets: two are kinases, as PI3K p110beta and PKC theta; others include Rack1/Gnb2l1, Erbin/Erbb2ip (an RTK modulator), two docking proteins (Gab2, Irs2), and Pleckstrin2. References are cited by PMID number.
Among twelve remaining EPO/EPOR modulated STF's (Table 1, including references as PMID's), one has been reported previously as insulin receptor substrate-2 (Irs2), a docking protein also utilized by the IL4R. Eleven represent novel EPO- response factors. One similarly is a docking protein, Gab2, while two are kinases as PI3K's catalytic beta subunit (Pik3cb), and PKC-theta (Prkcq) (an NFKb regulator). Another, MOB1 (Mobkl1a) is a preferred substrate of Mst1/2 Ste20- like kinases. Three are G- (or G-like) proteins (or binding proteins) as Gnb2l1, Gnl3 and Nol8; and three are regulating co-factors for RTKs, Toll receptors, and nuclear receptors as Erbin/Erbb2ip, Tirap, and Pnrc1, respectively. Finally, one EPO/EPOR response factor is a regulator of cytoskeleton restructuring as Pleckstrin-2 (Plek2) (3.9-fold induction). Thus, EPO also modulates the expression of intriguingly diverse sub-sets of novel STF's with functions that will be of significant interest to further delineate (and network).