Bulk RNA-seq of plasma-positive microglia and plasma-negative microglia from hypothalamus region in young mice

Microglia are highly responsive to their local environment and signals from the periphery. Blood plasma proteins readily permeate the healthy brain parenchyma. However, whether microglia are responsive to such proteins is unclear. Here, we identify a stable population of microglia takes up plasma proteins in a region-specific manner in healthy brains. To understand the difference of these two populations at the transcriptomic level, we sorted plasma-positive microglia and plasma-negative microglia from hypothalamus region of young mice (3-month-old). Bulk-RNAseq of sorted microglia from hypothalamus region of young mice 20 hours after injecting with dye-labeled plasma protein (n=4 replicates)