Airway Epithelial Transcriptome in Lung Transplant Recipients with Cystic Fibrosis and/or Pseudomonas

Lung transplant recipients with cystic fibrosis were found to tolerate Pseudomonas aeruginosa infection better than non-CF individuals. We hypothesized that differences in airway immune responses might relate to these more favorable outcomes. We prospectively collected airway brushes from lung transplant recipients with PsA+ cultures from 9 CF and 6 non-CF individuals. These were matched 1:1 with PsA- cases, and we performed next-generation RNA sequencing to evaluate the host transcriptome. RNA was sequenced using Illumina-based next generation sequencing and count data are supplied as a GEO-format count matrix. We also cultured airway epithelial cells and performed differential DNA methylation analysis using the Infinium HumanMethylation450 BeadChip. The data are included here as in EPIC report format.

肺移植接受者中囊性纤维化患者被发现对铜绿假单胞菌感染具有较非囊性纤维化个体更佳的耐受性。我们假设呼吸道免疫反应的差异可能与这些更有利的预后相关。我们前瞻性地收集了9名囊性纤维化患者和6名非囊性纤维化个体带有铜绿假单胞菌培养的肺移植接受者的呼吸道刷片，并与1:1配对的铜绿假单胞菌阴性病例进行匹配。我们对宿主转录组进行了下一代RNA测序分析。RNA测序采用基于Illumina的下一代测序技术，并作为GEO格式的计数矩阵提供计数数据。此外，我们还培养了呼吸道上皮细胞，并使用Infinium HumanMethylation450 BeadChip进行了差异DNA甲基化分析。数据以EPIC报告格式包含在此处。