Transcriptomic analysis of HexA-regulated gene expression in Pectobacterium carotovorum during different growth phases

HexA is a global transcriptional regulator involved in the modulation of virulence and environmental adaptation in Pectobacterium carotovorum. To identify the genes regulated by HexA, we performed RNA-seq analysis comparing the wild-type strain PCC27 and a hexA deletion mutant at mid-log and early stationary growth phases. This study provides comprehensive transcriptomic profiles that reveal the influence of HexA on gene expression dynamics across growth stages. Total RNA was extracted from P. carotovorum PCC27 wild-type and ΔhexA mutant strains grown in LB medium. Samples were collected at two time points: mid-log phase and early stationary phase. Three independent biological replicates were prepared for each condition. rRNA-depleted libraries were sequenced using the Illumina NovaSeq 6000 platform with a paired-end 2 × 101 bp configuration.