S3 Data -

Fig 5. LuxR regulation of methionine biosynthesis genes. (B–G) Relative transcript levels determined by RT-qPCR for cultures grown in M9GC medium (B–D) or M9G medium (E–G). Error bars show the mean and standard deviation of 3 biological replicates. (B–D) Different letters above the bars indicate that the pair-wise comparison is significantly different; the same letters above 2 bars indicate no significant differences (p < 0.05). One-way analysis of variance (ANOVA) was performed on log-normalized data (normally distributed, Shapiro–Wilk test; n = 3 biological replicates, Tukey’s multiple comparisons test). (E–G) Student’s–test was performed data (normally distributed, Shapiro–Wilk test; n = 3 biological replicates; p < 0.05, *; p < 0.001, ***). (H) Quantification of DPDQ (derivatization of DPD, the precursor to AI-2) via mass spectrometry from supernatants from strains grown in M9G medium and collected at HCD. DPDQ levels were normalized to an internal control (Verapamil) spiked in every sample. The mean of 3 independent biological replicates is shown.
(XLSX)