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454 sequencing of the MHC-Drb exon 2

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DataONE2015-06-25 更新2024-06-27 收录
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Raw sequence 454 data + primers with MID barcodes. We used a modified version of the primers LA31 (5’- GATCCTCTCTCTGCAGCACATTTCCT-3’) and LA32 (5’-TTCGCGTCACCTCGCCGCTG-3’) initially designed for cattle (Sigurdardottir et al. 1991), with the addition of individual-specific MIDS (Multiplex Identifiers) and adaptors required for emPCR and 454 sequencing.
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2015-06-25
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